Support, restriction enzymes: Description of Icons

Restriction Enzymes

Modifying Enzymes

PCR, qPCR, RT-PCR & dNTPs

Molecular Cloning

Nucleic Acid Purification

Molecular Labeling & Detection

In vitro Transcription

Electrophoresis Products

Nucleotides

Transfection Reagents

Reagents

Description of Icons

Fermentas restriction enzyme icons are designed as a guide for the selection of optimal conditions for your restriction digestion.

      Five Buffer System. Letters in the buffer icon indicate the buffer recommended for each restriction enzyme. They correspond to the codes of the Five Buffer System: B (blue), G (green), O (orange), R (red), Tango™ (yellow), respectively. Enzymes indicated by the “Unique” icon require a special buffer, which is supplied with the enzyme.

   Additives. Indicated additives should be used in the 1X reaction mixture to obtain the stated activity. Solutions of S-adenosylmethionine and oligonucleotides are supplied in separate vials. DTT (#R0861) is available separately.

     Incubation Temperature. Indicates the optimal incubation temperature in °Celsius.

      Ligation Efficiency. Indicates the ligation efficiency of DNA fragments generated by digestion with the restriction enzyme.

Star Activity. Restriction enzyme may exhibit star activity under the conditions described.

   Sensitive to Dam, Dcm Methylation or CpG Methylation. DNA cleavage by the restriction enzyme is blocked or impaired by Dam, Dcm or CpG methylation of the target sequences.

   Sensitive to Overlapping Dam, Dcm or CpG Methylation. The target site may be methylated in certain sequence contexts (overlapping methylation). This will result in blocked or impaired DNA cleavage.


Thermal Inactivation. Indicates thermal inactivation of the enzyme at 65°C or 80°C in 20 min.
Indicates that only small amounts of restriction enzyme (up to 10 units) can be inactivated at 80°C in 20 min.

High or Low Concentration. Indicates that the enzyme is available at a high or low concentrations.

Genome Qualified. Indicates that the restriction enzyme cleaves agarose-embedded DNA during megabase mapping of chromosomes. Handling of native DNA of this size in solution is often difficult due to double-stranded breaks introduced by mechanical DNA shearing. To avoid this problem, DNA is usually embedded into agarose plugs prior to digestion. Digested DNA is analyzed by pulsed field gel electrophoresis (PFGE).

Recombinant Enzyme. Indicates that the restriction enzyme has been purified from recombinant E.coli designed to overexpress this enzyme.

Blue/White Certified. Indicates that the restriction enzyme was tested by the Blue/White cloning assay.

FastDigest® Enzyme. Indicates the availability of a special formulation of the enzyme for fast digestion.

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Updated sausio 31, 2008 08:49

	Five Buffer System. Letters in the buffer icon indicate the buffer recommended for each restriction enzyme. They correspond to the codes of the Five Buffer System: B (blue), G (green), O (orange), R (red), Tango™ (yellow), respectively. Enzymes indicated by the “Unique” icon require a special buffer, which is supplied with the enzyme.
	Additives. Indicated additives should be used in the 1X reaction mixture to obtain the stated activity. Solutions of S-adenosylmethionine and oligonucleotides are supplied in separate vials. DTT (#R0861) is available separately.
	Incubation Temperature. Indicates the optimal incubation temperature in °Celsius.
	Ligation Efficiency. Indicates the ligation efficiency of DNA fragments generated by digestion with the restriction enzyme.
	Star Activity. Restriction enzyme may exhibit star activity under the conditions described.
	Sensitive to Dam, Dcm Methylation or CpG Methylation. DNA cleavage by the restriction enzyme is blocked or impaired by Dam, Dcm or CpG methylation of the target sequences.
	Sensitive to Overlapping Dam, Dcm or CpG Methylation. The target site may be methylated in certain sequence contexts (overlapping methylation). This will result in blocked or impaired DNA cleavage.
	Thermal Inactivation. Indicates thermal inactivation of the enzyme at 65°C or 80°C in 20 min. Indicates that only small amounts of restriction enzyme (up to 10 units) can be inactivated at 80°C in 20 min.
	High or Low Concentration. Indicates that the enzyme is available at a high or low concentrations.
	Genome Qualified. Indicates that the restriction enzyme cleaves agarose-embedded DNA during megabase mapping of chromosomes. Handling of native DNA of this size in solution is often difficult due to double-stranded breaks introduced by mechanical DNA shearing. To avoid this problem, DNA is usually embedded into agarose plugs prior to digestion. Digested DNA is analyzed by pulsed field gel electrophoresis (PFGE).
	Recombinant Enzyme. Indicates that the restriction enzyme has been purified from recombinant E.coli designed to overexpress this enzyme.
	Blue/White Certified. Indicates that the restriction enzyme was tested by the Blue/White cloning assay.
	FastDigest® Enzyme. Indicates the availability of a special formulation of the enzyme for fast digestion.