DraI
Available in FastDigest® format
5'-T T T^A A A-3' 3'-A A A^T T T-5'
digested lambda DNA
0.7% agarose#ER0221
Supplied with:
10X Buffer Tango™1500 u
1 ml#ER0222
Supplied with:
10X Buffer Tango™5x1500 u
1 ml#ER0223
Supplied with:
10X Buffer Tango™HC, 7500 u
1 mlCommercial Isoschizomers: - Related Documents (in pdf, ~55 KB):
Certificate of Analysis: #ER0221, #ER0222, #ER0223
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)Concentration
10 u/µl
50 u/µl, HCConditions for 100% Activity
Recommended
bufferIncubation
temperatureUnits for
overnight
incubation,
u/µg DNAThermal
inactivation,
in 20 minEnzyme activity, %
B
(blue)G
(green)O
(orange)R
(red)Tango™
(yellow)1X 1X 1X 1X 1X 2X Buffer Tango™ 37°C 0.1 65°C 50-100 50-100 20-50 20-50 100 50-100 Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton X-100, 0.2 mg/ml BSA and 50% glycerol.Ligation and Recleavage
After 50-fold overdigestion with DraI, more than 95% of the digested DNA fragments can be ligated and recut.
Methylation Effects Dam:
Dcm:
CpG:
EcoKI:
EcoBI:never overlaps
never overlaps
never overlaps
may overlap
never overlaps- no effect.
- no effect.
- no effect.
- blocked.
- no effect.Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.Double Digestions using Tango™ Buffer, DoubleDigest™
Optimal
bufferEnzyme activity, %
Tango™
(yellow)1X 2X Buffer Tango™ 100 50-100 Number of Recognition Sites in DNA Molecules
Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184 13 2 5 3 3 3 3 3 3 3 2
See also General Properties of Restriction Enzymes:
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Updated balandžio 22, 2008 11:22