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C A T A L O G
 

Eco88I (AvaI)
Available in FastDigest® format

5'-C^Py C G Pu G-3'
3'-G Pu G C Py^C-5'

digested lambda DNA
0.7% agarose
         
#ER0381
Supplied with:
10X Buffer Tango™		 1000 u
 
1 ml
Commercial Isoschizomers:
Ama87I, AvaIm, (BmeT110Im), BsiHKCI, BsoBIm
 Enzymes in parentheses have different cleavage specificities.
"m" indicates different sensitivity to methylation.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0381
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 37°C 0.2 65°C 100 50-100 0-20 0-20 100 20-50

Storage Buffer
 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Eco88I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 completely overlaps
 never overlaps
 never overlaps		 - no effect.
- no effect.
- cleavage impaired.
- no effect.
- no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
C^CCGGG - ApeI, BshTI, BsaWI, Cfr9I, Cfr10I, Kpn2I, NgoMIV, SgrAI.
C^TCGAG - SalI, SmoI, XhoI.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 20-50

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
8 1 2 1 1 1 1 2 2 2 1

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:20