Home  Contacts  Order  Catalog  Support
 Search  Alphabetical Index  Numerical Index
 Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification
 Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents
C A T A L O G
 

EcoRI
Available in FastDigest® format

5'-G^A A T T C-3'
3'-C T T A A^G-5'

digested lambda DNA
0.7% agarose
          
#ER0271
Supplied with:
10X Buffer EcoRI
10X Buffer Tango™		 5000 u
 
2x1 ml
1 ml
#ER0272
 Supplied with:
10X Buffer EcoRI
10X Buffer Tango™		 5x5000 u
 
5x1 ml
1 ml
#ER0273
 Supplied with:
10X Buffer EcoRI
10X Buffer Tango™		 HC,25000 u
 
5x1 ml
1 ml
#ER0275
 Supplied with:
10X Buffer EcoRI
10X Buffer Tango™		 10000 u
 
4x1 ml
1 ml
Commercial Isoschizomers: FunII

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0271, #ER0272, #ER0273, #ER0275
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl; 50 u/µl, HC

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
EcoRI
(unique)		 B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 1X 2X
Buffer EcoRI 37°C 0.2 65°C 100 0-20 NR 100 100* NR 100

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).
NR: buffer is not recommended, because of high star activity.

Storage Buffer
10 mM potassium phosphate (pH 7.4 at 25°C), 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA, 0.15% Triton X-100 and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with EcoRI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 may overlap
 never overlaps
 may overlap		 - no effect.
- no effect.
- cleavage impaired.
- no effect.
- no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
MfeI, MunI, TasI, XapI.

Note

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer EcoRI NR 100

NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
5 0 1 1 1 1 1 1 1 0 1

See also General Properties of Restriction Enzymes:

 

 Home  Search  Contacts  Order  Catalog  Support

Updated lapkričio 11, 2008 09:23