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SfiI
Available in FastDigest® format

5'-G G C C N N N N^N G G C C-3'
3'-C C G G N^N N N N C C G G-5'
digested Ad2 DNA
0.7% agarose
         
#ER1821
 Supplied with:
10X Buffer G
10X Buffer Tango™		 1000 u
 
1 ml
1 ml
Commercial Isoschizomers: -

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1821
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer G 50°C 0.2 80°C 50-100 100 20-50 0-20 100 0-20

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 300 mM NaCl, 10 mM MgCl2, 1 mM DTT, 0.15% Triton X-100, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with SfiI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 may overlap
 may overlap
 never overlaps
 never overlaps		 - no effect.
- cleavage impaired.
- cleavage impaired.
- no effect.
- no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note

  • Assayed using Adenovirus-2 DNA.

  • Incubation at 37°C results in 10% activity.

  • For cleavage with SfiI at least two copies of its recognition sequence are required.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer G 100 NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184 Ad2
0 0 0 0 0 0 0 0 0 0 0 3

See also General Properties of Restriction Enzymes:

 

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Updated sausio 26, 2009 13:07