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Eco47I (AvaII)
Available in FastDigest® format

T 5'-G^G A C C-3' 3'-C C T G^G-5' A		digested lambda DNA 1.0% agarose
#ER0311 Supplied with: 10X Buffer R 10X Buffer Tango™	800 u   1 ml 1 ml
#ER0312 Supplied with: 10X Buffer R 10X Buffer Tango™	4000 u   2x1 ml 1 ml
Commercial Isoschizomers: AvaII, Bme18I, SinI, VpaK11BI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0311, #ER0312 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	37°C	0.3	65°C	0-20	50-100	50-100	100	50-100	50-100

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Eco47I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  may overlap  may overlap  never overlaps  never overlaps	- no effect. - blocked. - blocked. - no effect. - no effect.

Compatible Ends
Cfr13I, CpoI, PpuMI, Psp5II, SanDI, Sau96I.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer R	50-100	50-100

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
35	1	1	8	2	2	2	2	2	4	5

Updated balandžio 22, 2008 11:21