Cfr9I (XmaI*)
5'-C^C C G G G-3' 3'-G G G C C^C-5'
digested lambda DNA
0.7% agarose#ER0171
Supplied with:
10X Buffer Cfr9I
10X Buffer Tango™300 u
1 ml
1 ml#ER0172
Supplied with:
10X Buffer Cfr9I
10X Buffer Tango™1500 u
1 ml
1 mlCommercial Isoschizomers:
(SmaIm), (FastDigest® SmaIm), TspMIm, XmaCI, XmaIm
Enzymes in parentheses have different cleavage specificities.
"m" indicates different sensitivity to methylation.Related Documents (in pdf, ~55 KB):
Certificate of Analysis: #ER0171, #ER0172
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)Concentration
10 u/µlConditions for 100% Activity
Recommended
bufferIncubation
temperatureUnits for
overnight
incubation,
u/µg DNAThermal
inactivation,
in 20 minEnzyme activity, %
Cfr9I
(unique)B
(blue)G
(green)O
(orange)R
(red)Tango™
(yellow)1X 1X 1X 1X 1X 1X 2X Buffer Cfr9I 37°C 0.2 65°C 100 0-20 0-20 0-20 0-20 20-50 0-20 Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 250 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.Ligation and Recleavage
After 50-fold overdigestion with Cfr9I, more than 95% of the digested DNA fragments can be ligated and recut.
Methylation Effects Dam:
Dcm:
CpG:
EcoKI:
EcoBI:never overlaps
never overlaps
completely overlaps
never overlaps
never overlaps- no effect.
- no effect.
- cleavage impaired.
- no effect.
- no effect.Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.Note
To achieve complete digestion of substrate with Cfr9I, the concentration of DNA should be no less than 50 µg/ml in the reaction buffer.Double Digestions using Tango™ Buffer, DoubleDigest™
Optimal
bufferEnzyme activity, %
Tango™
(yellow)1X 2X Buffer Cfr9I 20-50 NR NR: buffer is not recommended, since enzyme activity is less than 20%.
Number of Recognition Sites in DNA Molecules
Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184 3 0 1 0 1 1 1 1 1 1 0
See also General Properties of Restriction Enzymes:
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Updated spalio 27, 2008 11:26