FastDigest® EagI (Eco52I)*
* FastDigest® EagI (Eco52I) is a proprietary formulation of Eco52I,
an isoschizomer of EagI having the same recognition and
cleavage specificity.5'-C^G G C C G-3' 3'-G C C G G^C-5'
0.7% agarose
Digested lambda DNA in 5 min with FastDigest® EagI (Eco52I)
fast digest, fast digestion, time saver#FD0334
Supplied with:
10X FastDigest® buffer50 reactions
1 mlRelated Documents (in pdf, ~55 KB):
Certificate of Analysis: #FD0334
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 834 KB)
Brochure (in pdf, 383 KB)
LabAid™ (in pdf, 97 KB)
Features
- All FastDigest® enzymes work in the same reaction conditions.
- Single and double digestion of DNA in only 5 min.
- No star activity in prolonged incubations.
- Enhanced performance in one-hour DNA cleavage reactions.
Unit Definition
One FastDigest® Unit (FDU) is the amount of the enzyme required to cleave 1 µg of lambda DNA-Eco81I fragments in 5 min at 37°C in 1X FastDigest® buffer.Concentration
1 FDU/µl.
Recommended
bufferReaction conditions
Temperature Time FastDigest® buffer 37°C 5 min Thermal Inactivation
FastDigest® EagI (Eco52I) is inactivated by incubation at 65°C for 5 min.Digestion of Plasmid DNA
1 µl of FastDigest® EagI (Eco52I) digests up to 1 µg of plasmid DNA in 20 min, see Protocol for Fast Digestion and Analysis of Plasmid DNA with FastDigest® Enzymes and Protocol for Fast Vector Preparation with FastDigest® Enzymes.Digestion of PCR Products
1 µl of FastDigest® EagI (Eco52I) digests ~0.2 µg of PCR product in 20 min, see Protocol for Fast Digestion of PCR Products with FastDigest® Enzymes.Digestion of Genomic DNA
1 µl of FastDigest® EagI (Eco52I) digests 1 µg of genomic DNA in 5 min, or 5 µg of genomic DNA in 30 min, see Protocol for Fast Digestion of Genomic DNA with FastDigest® Enzymes.Ligation and Recleavage
After overdigestion with 1 µl of FastDigest® EagI (Eco52I) for 1 hour, more than 95% of the digested DNA fragments can be ligated and recut.
Methylation Effects Dam:
Dcm:
CpG:
EcoKI:
EcoBI:never overlaps
never overlaps
completely overlaps
never overlaps
never overlaps- no effect.
- no effect.
- blocked.
- no effect.
- no effect.Recommendations for Double and Triple Digestions with FastDigest® Enzymes
Perform 5 minute double or triple digestions with FastDigest® EagI (Eco52I) and any other FastDigest® enzyme(s) in FastDigest® buffer, see Protocol for Double Digestion with FastDigest® Enzymes and Protocol for Triple Digestion with FastDigest® Enzymes.
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Updated rugsėjo 03, 2008 12:35