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pUC18
GenBank/EMBL accession number L09136.pUC19
GenBank/EMBL accession number L09137.Note
Indicates restriction sites of Fermentas enzymes that are sensitive (cleavage completely blocked or partially inhibited) to overlapping Dam/Dcm methylation.
- Complete cleavage is achieved with the DNA substrates isolated from dam- or dcm- hosts.
There are no restriction sites in pUC18 DNA for the following enzymes:
AarI, AdeI, AlfI, AloI, ApaI, BaeI, BbvCI, BclI, BcuI, BglII, BoxI, BpiI, BplI, Bpu10I, Bpu1102I, BsaAI, BseJI, BseRI, BsgI, BshTI, Bsp68I, Bsp119I, Bsp120I, Bsp1407I, BspTI, Bst1107I, BstXI, Bsu15I, BtgI, BtrI, Cfr42I, CpoI, Eco32I, Eco47III, Eco52I, Eco72I, Eco81I, Eco91I, Eco105I, Eco130I, Eco147I, FalI, FaqI, FseI, FspAI, Kpn2I, KspAI, MlsI, MluI, Mph1103I, MssI, MunI, Mva1269I, NcoI, NheI, NotI, OliI, PacI, PauI, PdiI, Pfl23II, PsiI, Psp5II, PsrI, PsyI, SanDI, SexAI, SfiI, SgfI, SgrAI, SgsI, SmiI, SrfI, TaqII, TstI, Van91I, XagI, XcmI, XhoI, XmaJI.A B C D E H K M N P R S X
Enzyme Number of recognition sites
1 2 3 4 5 6 7 8 9 10 11 12 AasI 91 908 AatII 2617 Acc65I 438 AflIII 806 Alw21I 177 444 1120 2281 2366 Alw26I 51 1766 2531 2684 Alw44I 177 1120 2366 BamHI 429 BccI 1735 1859 2146 BceAI 387 1292 BcgI 2215 BcnI 47 82 434 435 1185 1881 2232 BfiI 364 1744 BfmI 411 1071 1262 1940 BfuI 1015 2542 BglI 245 1813 Bme1390I 47 82 354 434 435 545 833 954 967 1185 1881 2232 BpuEI 912 1174 1451 2319 BsaWI 1012 1159 1990 BsaXI 659 BseDI 354 433 434 545 966 BseGI 77 321 1679 1860 2147 BseLI 47 648 822 840 1006 1285 BseMI 1753 1935 BseMII 171 1081 1490 1656 2196 BseNI 365 391 606 1209 1222 1339 1745 1863 1906 2170 2345 BseSI 177 1120 2366 BseXI 41 254 327 630 711 729 1148 1213 1216 1422 1750 2116 BseYI 1110 Bsh1236I 2 4 107 652 654 852 1433 1763 2256 2588 Bsh1285I 276 719 1143 2066 2215 BshNI 235 438 550 1647 BspLI 235 429 438 550 836 875 1647 1741 1782 1993 2583 BspPI 429 430 1373 1447 1459 1544 1557 2021 2324 2342 BssSI 979 2363 2670 BsuRI 287 389 646 820 831 849 1283 1741 1821 2088 2675 BstAPI 179 BtsI 593 2092 2120 BveI 413 CaiI 1217 CfrI 388 645 2087 Cfr9I 434 Cfr10I 1779 Cfr13I 286 1741 1820 1837 2059 2675 CseI 108 908 1486 2236 Csp6I 168 439 2178 DraI 1563 1582 2274 Eam1104I 290 684 2488 Eam1105I 1694 EciI 878 1024 1852 Ecl136II 444 Eco24I 444 Eco31I 1766 Eco47I 1837 2059 Eco57I 1333 2381 Eco57MI 1333 1784 2381 Eco88I 434 EcoO109I 2674 EcoRI 450 EcoRII 354 545 833 954 967 EheI 235 Esp3I 51 2683 FspBI 424 1301 1554 1889 GsuI 1784 Hin1I 235 2235 2617 Hin1II 38 406 461 807 1527 2018 2028 2106 2142 2535 2640 Hin4I 1694 1768 HincII 417 HindIII 399 HinfI 420 641 706 781 1177 1694 HphI 12 21 1550 1777 2173 2399 2414 Hpy8I 177 417 1120 1608 2366 Hpy99I 372 385 907 1701 1964 Hpy188I 31 156 918 996 1349 1483 1618 2064 2075 2195 HpyF3I 171 1081 1490 1656 2196 2622 KpnI 438 LweI 78 153 208 229 894 1946 2156 2386 MaeIII 57 348 368 1163 1226 1342 1625 1956 2014 2167 2355 MbiI 496 737 2538 MboII 291 685 1304 1456 1547 2302 2380 2489 MmeI 996 1180 MslI 1947 2106 2465 MspA1I 112 306 628 1146 1391 2332 MvaI 354 545 833 954 967 NdeI 183 NmuCI 57 368 1956 2167 NsbI 256 1919 PaeI 405 PagI 1526 2534 2639 PdmI 2294 PfeI 641 781 PfoI 46 PpiI 1497 2340 PscI 806 Psp1406I 1924 2297 PsuI 429 1447 1458 1544 1556 2324 2341 PstI 411 PvuI 276 2066 PvuII 306 628 RsaI 168 439 2178 SacI 444 SalI 417 SapI 683 ScaI 2177 SchI 420 706 1177 1694 SdaI 410 SduI 177 444 1120 2281 2366 SmaI 434 SmlI 912 1174 1451 2319 SmuI 114 124 284 598 655 SspI 2501 TaaI 19 54 262 768 839 1309 1622 2137 TaiI 374 1509 1925 2298 2618 TaqI 418 448 906 2350 TasI 451 487 504 579 1567 1873 2128 TatI 167 2177 TauI 150 732 850 1005 2089 2211 2440 TseI 41 254 327 630 711 729 1148 1213 1216 1422 1750 2116 TspDTI 639 1575 1677 1980 TspGWI 2149 2466 VspI 576 635 1870 XapI 450 XbaI 423 XceI 37 405 806 XmiI 417
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Updated liepos 25, 2008 10:12
